Hi, if you've paid for tuition and others and want a refund from your college or university, then you should submit proof of visa denial. Normally, one is not required to pay tuition in advance. You chose to in order to support your visa application?
Anyway, you're entitle to refund as far as deadline for refunds hasn't passed and the university/college rules allow for that. However, if you want refund while your visa application is under process, it's advisable you withdraw your visa application before applying for refund because you might have sent a proof of tuition payment in support of your application. In this way, you're save if you decide to apply later or in the near future for visa to Canada.