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My Husband is in layoff but employee given LMIA. Will this affect our CSQ and we are getting EI now.

Assume you mean employer got LMIA. Have you updated you application to indicate that you are no longer working for the employer? Did you use the job offer to qualify for PR?’ if you are talking about LMIA your spouse must be on a closed work permit. If that is the case your spouse shouldn’t qualify for EI because on a closed work permit you are unable to be ready to work and are looking for work. If you were on an open WP then you could qualify for EI.

https://www.canada.ca/en/services/benefits/ei/ei-regular-benefit/eligibility.html